Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 23, Issue 12, Pages 1291-1299Publisher
WILEY
DOI: 10.1002/bmc.1252
Keywords
beta-adrenergic blockers; chiral separation; RP-HPLC; chiral variants of Sanger's reagent; dinitrophenyl-L-Pro-N-hydroxysuccinimide ester; N-succinimidyl-(S)-2-(6-methoxynaphth-2-yl) propionate
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Funding
- University Grants Commission (UGC) New Delhi, India
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Twelve chiral derivatizing reagents (CDRs) were synthesized by substituting one of the fluorine atoms in 1,5-difluoro-2,4-dinitrobenzene (DFDNB) with three optically pure amines [(R)-(-)-1-cyclohexylethylamine, (+)-dehydroabietylamine and (S)-(-)-alpha,4-dimethylbenzylamine], six amino acid amides [L-Ala-NH2, L-Phe-NH2, L-Val-NH2, L-Leu-NH2, L-Met-NH2 and D-Phg-NH2] and three amino acids [L-Ala, L-Val and L-Leu]. In addition, dinitrophenyl-L-Pro-N-hydroxysuccinimide ester and N-succinimidyl-(S)-2-(6-methoxynaphth-2-yl) propionate were also synthesized and used as CDR. Keeping in view the presence of an amino group, diastereomers of six beta-blockers (atenolol, propranolol, bisoprolol, metoprolol, salbutamol, and carvedilol) were synthesized by reaction with these 14 CDRs. The diastereomers were separated by RP-HPLC. The method was validated for linearity, accuracy, limit of detection and limit of quantification. Copyright (C) 2009 John Wiley & Sons, Ltd.
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