Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 196, Issue 2, Pages 147-149Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-5173(99)00409-3
Keywords
nanoparticles; gelatin; covalent linkage; avidin; peptide nucleic acid (PNA)
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The possibility of preparing uniform nanoparticles consisting of proteins such as gelatin followed by covalent linkage of avidin was investigated. Gelatin nanoparticles were prepared by two step desolvation. Functional groups at the surface of the particulate system were quantified with site-specific reagents. The surface of the nanoparticles was thiolated and avidin was covalently attached to the nanoparticles via a bifunctional spacer at high levels. Biotinylated peptide nucleic acid (PNA) was effectively complexed by the avidin-conjugated nanoparticles. Avidin-conjugated protein nanoparticles should prove as potential carrier system for biotinylated drug derivatives in antisense therapy. (C) 2000 Elsevier Science B.V. All rights reserved.
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