Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 97, Issue 6, Pages 2556-2561Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.050588497
Keywords
-
Categories
Funding
- NIGMS NIH HHS [R01 GM055632, GM54179, R01 GM054179] Funding Source: Medline
- NINDS NIH HHS [R01 NS036706, NS367060] Funding Source: Medline
Ask authors/readers for more resources
Communication-incompetent cell lines were transfected with connexin (Cx) 43 fused with enhanced green fluorescent protein (EGFP) to examine the relation between Cx distribution determined by fluorescence microscopy and electrical coupling measured at single-channel resolution in living cell pairs, Cx43-EGFP channel properties were like those of wild-type Cx43 except for reduced sensitivity to transjunctional voltage. Cx43-EGFP clustered into plaques at locations of cell-cell contact. Coupling was always absent in the absence of plaques and even in the presence of small plaques. Plaques exceeding several hundred channels always conferred coupling, but only a small fraction of channels were functional, These data indicate that clustering may be a requirement for opening of gap junction channels.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available