Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 28, Issue 6, Pages 846-853Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0891-5849(99)00280-4
Keywords
carbamoyl-PROXYL; ESR-CT; ESR imaging; ESR measurement; free radical; oxidative stress
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Real-time detection of free radicals generated within the body may contribute to clarify the pathophysiological role of free radicals in disease processes. Of the techniques available for studying the generation of free radicals in biological systems, electron spin resonance (ESR) has emerged as a powerful tool for detection and identification. This article begins with a review of spin trapping detection of oxygen-centered radicals using X-band ESR spectroscopy and then describes the detection of superoxide and hydroxyl radicals by the spin trap 5,5-dimethyl-1-pyrroline-N-oxide and ESR spectroscopy in the perfusate from isolated perfused rat livers subjected to ischemia/reperfusion. This article also reviews the current status of ESR for the in vivo detection of free radicals and in vivo imaging of exogenously administered free radicals. Moreover, we show that in vivo ESR-computed tomography with 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl may be useful for noninvasive anatomical imaging and also for imaging of hepatic oxidative stress in vivo. (C) 2000 Elsevier Science Inc.
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