Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 27, Issue 1, Pages 99-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0141-8130(99)00123-3
Keywords
beetle larva cuticle; silkworm exuvia; chitin; chitosan; alpha-form structure; degree of crystallinity
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Chitin samples in a cl-form structure were isolated from beetle larva cuticle and silkworm (Bombyx mori) pupa exuvia by treatment with 1 N HCl and 1 N NaOH. Chitosan was prepared by treating them in 40% NaOH containing NaBH4. Chitin and chitosan were analyzed by X-ray, [C-13]CP/MAS NMR, [C-13]FT-NMR, and scanning electron microscopy (SEM) methods. Insect chitin degraded more readily than shrimp chitin when treated with 6 N HCl and the enzyme-chitinase. After treatment with 2 N HCl at 100 degrees C, the insect chitin crystallinity increased. N-deacetylation of insect chitin was easier than that of crustaceous chitin, and about 94% of the N-acetyl groups were removed in one treatment with 40% NaOH for 4 h at 110 degrees C. After treatment with 2 N HCl, 55% of the N-acetyl groups of silkworm chitin were removed under the same conditions. Beetle chitin showed a higher affinity for chitinase than shrimp chitin. (C) 2000 Elsevier Science B.V. All rights reserved.
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