Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 275, Issue 11, Pages 7701-7707Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.275.11.7701
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Funding
- NHLBI NIH HHS [HL 29282, HL 53590] Funding Source: Medline
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Proteoglycan-binding peptides were designed based on consensus sequences in heparin-binding proteins: XBBXBX and XBBBXXBX, where X and B are hydropathic and basic residues, respectively. initial peptide constructs included (AKKARA)(n) and (ARKKAAKA)(n) (n = 1-6), Affinity coelectrophoresis revealed that low M-r peptides (600-1300) had no affinities for low M-r heparin, but higher M-r peptides (2000-3500) exhibited significant affinities (K-d congruent to 50-150 nM), which increased with peptide M-r. Affinity was strongest when sequence arrays were contiguous and alanines and arginines occupied hydropathic and basic positions, but inclusion of prolines was disruptive. A peptide including a single consensus sequence of the serglycin proteoglycan core protein bound heparin strongly (K-d congruent to 200 nM), likely owing to dimerization through cysteine-cysteine linkages. circular dichroism showed that high affinity heparin-binding peptides converted from a charged coil to an alpha-helix upon heparin addition, whereas weak heparin-binding peptides did not. Higher M-r peptides exhibited high affinities for total endothelial cell proteoglycans (K-d congruent to 300 nM), and similar to 4-fold weaker affinities for their free glycosaminoglycan chains. Thus, peptides including concatamers of heparin-binding consensus sequences may exhibit strong affinities for heparin and proteoglycans. Such peptides may be applicable in promoting cell-substratum adhesion or in the design of drugs targeted to proteoglycan-containing cell surfaces and extracellular matrices.
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