Journal
NEUROSCIENCE LETTERS
Volume 282, Issue 1-2, Pages 1-4Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(00)00817-X
Keywords
iron; oxidation; free radical; central nervous system hemorrhage; stroke; cell culture
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Exposure to micromolar concentrations of hemoglobin (Hb) results in the oxidative death of cultured cortical neurons, but glia are resistant. The role of heme oxygenase-1 (HO-1) induction on this glial resistance was investigated. Within two hours of exposure to 5 mu M Hb, immunoblotting demonstrated an increase in HO-1 in confluent glial cultures. Consistent with prior observations, 23-30 h Hb exposure had little or no effect on glial viability, as assessed by lactate dehydrogenase release. Concomitant treatment with the HO inhibitors tin protoporphyrin IX or the D-amino acid peptide rvnlrialry resulted in release of 40-71% of glial lactate dehydrogenase; protein synthesis inhibition with cycloheximide produced a similar effect. These results are consistent with the hypothesis that HO-1 induction protects cortical astrocytes from Hb toxicity. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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