Journal
FEBS LETTERS
Volume 470, Issue 1, Pages 55-60Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01287-4
Keywords
islet amyloid polypeptide; fibril; beta-sheet; amyloid
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Human synthetic islet amyloid polypeptide (hIAPP) is rapidly converted to beta-sheet conformation and fibrils in aqueous media. Optimal solubility conditions for hIAPP were determined by circular dichroism spectroscopy and transmission electron microscopy, hIAPP in trifluoroethanol or hexafluoro-2-isopropanol (HFIP) diluted in water or phosphate buffer (PB) exhibited random structure which was converted to beta-sheet and fibrils with time, hIAPP, solubilised in HFIP, filtered and lyophilised remained in stable random structure for up to 7 days in water; in PB, insoluble aggregates precipitated from which protofilaments and fibrils formed with time. This suggests that amorphous aggregates of hIAPP could initiate islet amyloidosis in vivo. (C) 2000 Federation of European Biochemical Societies.
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