Journal
NEUROREPORT
Volume 11, Issue 4, Pages 709-712Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200003200-00011
Keywords
adenosine; cerebellar granule cells; ecto-nucleotidase; glutamate
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Several enzymes hydrolyze ATP, producing ADP which is hydrolyzed to AMP. Ecto-5'-nucleotidase produces adenosine from AMP. Glutamate (Glu) is an excitatory neurotransmitter and increases extracellular adenosine levels, which is considered an important inhibitory neuromodulator. Here we show that Glu activates ADP and AMP hydrolysis. NMDA and kainic acid (KA) also increased these enzymatic activities, but 1-aminocyclopentane-1S,3R-dicarboxylic acid (ACPD) had no effect. Dihydrokainate (DHK), an inhibitor of glutamate uptake, also blocked glutamate-evoked activation of ecto-nucleotidases, suggesting that this activation was also Glu transporters dependent. Therefore, we suggest that the Glu-evoked stimulation of ecto-nucleotidases might contribute to the increase of adenosine in extracellular space induced by Glu. NeuroReport 11:709-712 (C) 2000 Lippincott Williams & Wilkins.
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