Journal
BIOMATERIALS
Volume 35, Issue 9, Pages 2942-2951Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.12.007
Keywords
Quantum dots; Hematocyte invasion; Hematopoiesis damage; In vivo test
Funding
- National High-Tech R&D Program of China (863 Program) [2011AA100306]
- National Basic Research Program of China (973 Program) [2013CB934400, 2012CB932400]
- NSFC [61361160412, 30900338]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Provincial Key Technology R&D Program of Jiangsu [BE2011327-1]
- Qing Lan Project for Excellent Scientific and Technological Innovation Team of Jiangsu Province
- Project for Jiangsu Scientific and Technological Innovation Team
Ask authors/readers for more resources
Quantum dots (QDs) have gained significant attention due to their superior optical properties and wide usage in biological and biomedical studies. In recent years, there has been intense concern regarding the in vivo toxicity of QDs. This study was undertaken to examine the toxicity of CdTe QDs on hematopoiesis in an invertebrate model organism, Bombyx mori. Vascular injection of sub-lethal doses of QDs in B. mori larvae caused time- and dose-dependent damage in the hematopoietic organ and hematocytes. QDs with the maximum emission wavelength of 530 nm (QDs530) were quickly observed in cystocytes and plasmacytes, and gradually bleached their green fluorescence, followed by a decrease in peripheral hematocytes. Additionally, the proportion of abnormal hematocytes increased. In marked contrast, QDs with the maximum emission wavelength of 720 nm (QDs720) were quickly surrounded by hematocytes and subsequently enriched in cystocytes like the human's leukocytes, but with weaker cytotoxicity. QDs exposure promoted the mitotic nucleus in prohemocytes and hematocytes similar to peripheral blood stem cells in humans, but aggravated apoptosis. A decrease in hematopoiesis was accompanied by shrinkage and death of hematopoietic organs via an increase in reactive oxygen species. QDs with smaller size resulted in more severe hematopoiesis toxicity. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available