Journal
BIOMATERIALS
Volume 35, Issue 37, Pages 9811-9823Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.09.002
Keywords
Paper scaffold; Initiated chemical vapor deposition; Human adipose-derived stem cell; Osteogenic differentiation; Bone tissue engineering
Funding
- Korea Health Technology R&D Project - Ministry of Health and Welfare, Republic of Korea [HI13C1479]
- Translational Research Center for Protein Function Control (TRCP) - Ministry of Science, ICT and Future Planning (MSIP), Republic of Korea [2009-0083522]
- Advanced Biomass R&D Center (ABC) of the Global Frontier Project - Ministry of Education, Science and Technology [ABC-2011-0031356]
- National Agenda Project (NAP) - Korea Research Council of Fundamental Science Technology [NAP-09-04]
- National Research Council of Science & Technology (NST), Republic of Korea [NAP-09-04] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a water-repellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques. An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. To the best of our knowledge, this is the first study reporting the feasibility of a paper material for stem cell application to repair tissue defects. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available