Journal
BIOMATERIALS
Volume 35, Issue 27, Pages 7762-7773Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.06.011
Keywords
Cancer stem-like cells; Miniaturized 3D culture; Alginate; Hydrogel; Microcapsule
Funding
- American Cancer Society (ACS) Research Scholar Grant [120936-RSG-11-109-01-CDD]
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Cancer stem-like cells (CSCs) are rare subpopulations of cancer cells that are reported to be responsible for cancer resistance and metastasis associated with conventional cancer therapies. Therefore, effective enrichment/culture of CSCs is of importance to both the understanding and treatment of cancer. However, it usually takes approximately 10 days for the widely used conventional approach to enrich CSCs through the formation of CSC-containing aggregates. Here we report the time can be shortened to 2 days while obtaining prostate CSC-containing aggregates with better quality based on the expression of surface receptor markers, dye exclusion, gene and protein expression, and in vivo tumorigenicity. This is achieved by encapsulating and culturing human prostate cancer cells in the miniaturized 3D liquid core of microcapsules with an alginate hydrogel shell. The miniaturized 3D culture in core-shell microcapsules is an effective strategy for enriching/culturing CSCs in vitro to facilitate cancer research and therapy development. (C) 2014 Elsevier Ltd. All rights reserved.
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