Journal
BIOMATERIALS
Volume 35, Issue 26, Pages 7363-7373Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.05.019
Keywords
Biomimetic material; Collagen; Cell encapsulation; ECM (extracellular matrix); Growth factor; RGD peptide
Funding
- National Science Foundation [1213948-CHE]
- TRASK Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1213948] Funding Source: National Science Foundation
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Natural and synthetic three-dimensional (3-D) scaffolds that mimic the microenvironment of the extracellular matrix (ECM), with growth factor storage/release and the display of cell adhesion signals, offer numerous advantages for regenerative medicine and in vitro morphogenesis and oncogenesis modeling. Here we report the design of collagen mimetic peptides (CMPs) that assemble into a highly crosslinked 3-D matrix in response to metal ion stimuli, that may be functionalized with His-tagged cargoes, such as green fluorescent protein (GFP-His(8)) and human epidermal growth factor (hEGF-His(6)). The bound hEGF-His(6) was found to gradually release from the matrix in vitro and induce cell proliferation in the EGF-dependent cell line MCF10A. The additional incorporation of a cell adhesion sequence (RGDS) at the N-terminus of the CMP creates an environment that facilitated the organization of matrix-encapsulated MCF10A cells into spheroid structures, thus mimicking the ECM environment. (C) 2014 Elsevier Ltd. All rights reserved.
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