Journal
BIOMATERIALS
Volume 35, Issue 24, Pages 6182-6194Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.04.067
Keywords
Carbon nanotubes; Nanoparticles; Platelets; Biocompatibility; Nanotoxicity; Protein corona
Funding
- U.S. Department of Energy
- U.S. Food and Drug Administration
- Ministry of Education, Youth and Sports of Czech Republic [LH12014]
- Charles University in Prague [PRVOUK-P24/LF1/3]
- FDA CORES
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Carbon nanotubes (CNT) are one of the most promising nanomaterials for use in medicine. The blood biocompatibility of CNT is a critical safety issue. In the bloodstream, proteins bind to CNT through non-covalent interactions to form a protein corona, thereby largely defining the biological properties of the CNT. Here, we characterize the interactions of carboxylated-multiwalled carbon nanotubes (CNTCOOH) with common human proteins and investigate the effect of the different protein coronas on the interaction of CNTCOOH with human blood platelets (PLT). Molecular modeling and different photophysical techniques were employed to characterize the binding of albumin (HSA), fibrinogen (FBG), gamma-globulins (IgG) and histone H1 (H1) on CNTCOOH. We found that the identity of protein forming the corona greatly affects the outcome of CNTCOOH's interaction with blood PLT. Bare CNTCOOH-induced PLT aggregation and the release of platelet membrane microparticles (PMP). HSA corona attenuated the PLT aggregating activity of CNTCOOH, while FBG caused the agglomeration of CNTCOOH nanomaterial, thereby diminishing the effect of CNTCOOH on PLT. In contrast, the IgG corona caused PLT fragmentation, and the NI corona induced a strong PLT aggregation, thus potentiating the release of PMP. Published by Elsevier Ltd.
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