Journal
BIOMATERIALS
Volume 34, Issue 21, Pages 5254-5261Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.03.024
Keywords
Graphene oxide; PVP; Dendritic cells; T lymphocytes; Macrophages; Immunotoxicity
Funding
- National Key Basic Research Program (973 Project) [2010CB933901]
- National Natural Scientific Fund [81225010, 31100717]
- Shanghai Science and Technology Fund [10XD1406100]
- Special Project for Nanotechnology from Shanghai [1052nm04100]
- Shanghai Jiao Tong University Innovation Fund [AE340201]
- Shanghai Jiao Tong University Investment Fund
- Ministry of Education
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Graphene oxide (GO) immunotoxicity is not clarified well up to date. Herein we reported the effects of GOs with and without polyvinylpyrrolidone (PVP) coating on human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages. Human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages were isolated from health donated bloods, PVP-coating GO (PVP-GO) exhibited lower immunogenicity compared with pure GO on the aspect of inducing differentiation and maturation of dendritic cells (DCs), the levels of secreted TNF-alpha. and IL-1 beta had no obvious difference between two groups, yet the secretion of IL-6 remained in PVP-coating GO group. In addition, PVP-coating GO delayed significantly the apoptotic process of T lymphocytes, at the same time, and exhibited anti-phagocytosis ability against macrophages and markedly enhanced the physiological activity of macrophages. In conclusion, PVP-coating GO possesses good immunological biocompatibility and immunoenhancement effects in vitro, and is likely to be an available candidate of immunoadjuvant in the future. (C) 2013 Elsevier Ltd. All rights reserved.
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