Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 275, Issue 13, Pages 9278-9283Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.275.13.9278
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- NIGMS NIH HHS [GM52825] Funding Source: Medline
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The p53 tumor suppressor protein is stabilized in response to ionizing radiation and accumulates in the nucleus. Stabilization is thought to involve disruption of the interaction between the p53 protein and Mdm2, which targets p53 for degradation. Here we show that the direct association between a p53 N-terminal peptide and Mdm2 is disrupted by phosphorylation of the peptide on Thr's but not by phosphorylation at other N-terminal sites, including Ser(15) and Ser(37). Thr(18) was phosphorylated in vitro by casein kinase (CK1); this process required the prior phosphorylation of Ser's Thr's was phosphorylated in vivo in response to DNA damage, and such phosphorylation required Ser's. Our results suggest that stabilization of p53 after ionizing radiation may result, in part, from an inhibition of Mdm(2) binding through a phosphorylation-phosphorylation cascade involving DNA damage-activated phosphorylation of p53 Ser(15) followed by phosphorylation of Thr(18).
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