Journal
BIOMATERIALS
Volume 34, Issue 16, Pages 4057-4067Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.02.057
Keywords
Airway tissue engineering; Transplantation; Bioartifical trachea; Cell proliferation assay; Cell viability; Synthetic scaffold
Funding
- Knut & Alice Wallenberg foundation
- Swedish Research Council
- Center for Biosciences at Karolinska Institutet
- European Project FP7-NMP- SMALL-5: BIOtrachea, Biomaterials for Tracheal Replacement in Age-related Cancer via a Humanly Engineered Airway [280584-2]
- ALF medicine (Stockholm County Council): Transplantation of bioengineered trachea in humans [LS1101-0042]
- Swedish Heart-Lung Foundation: Trachea tissue engineering
- Doctor Dorka Stiftung (Hannover, Germany): bioengineering of tracheal tissue
- Russian Ministry of Education and Science [11.G34.31.0065]
Ask authors/readers for more resources
The clinical outcome of transplantations of bioartificial tissues and organs depends on the presence of living cells. There are still no standard operative protocols that are simple, fast and reliable for confirming the presence of viable cells on bioartificial scaffolds prior to transplantation. By using mathematical modeling, we have developed a calorimetric-based system (calorimetric scale bar) to predict the cell viability and density for sufficient surface coverage. First, we refined a method which can provide information about cell viability and numbers in an in vitro setting: i) immunohistological staining by Phalloidin/DAPI and a modified calorimetric cell viability assay. These laboratory-based methods and the developed calorimetric-based system were then validated in rat transplantation studies of unseeded and seeded tracheal grafts. This was done to provide critical information on whether the graft would be suitable for transplantation or if additional cell seeding was necessary. The potential clinical impact of the calorimetric scale bar was confirmed using patient samples. In conclusion, we have developed a robust, fast and reproducible calorimetric tool that can verify and warrant viability and integrity of an engineered tissue/organ prior to transplantation. This should facilitate a successful transplantation outcome and ensure patient safety. (c) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available