Journal
BIOMATERIALS
Volume 34, Issue 12, Pages 3010-3019Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.01.022
Keywords
MRI; hMSCs; Fe3O4@SiO2; Single cell sensitivity
Funding
- Strategic Priority Research Program of Chinese Academy of Sciences [XDA01030201, XDA01030203]
- Ministry of Science and Technology of China [2011CB965004]
- Chinese Academy of Sciences
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Fe3O4@SiO2 core-shell nanoparticles were synthesized and used to label human mesenchymal stem cells (hMSCs) for in vitro and in vivo magnetic resonance imaging study. The diameter of the nanoparticles is 24-30 nm with a Fe3O4 core of similar to 8 nm and a SiO2 shell of similar to 8 nm. Transverse relaxivity of the nanoparticles dispersed in water is measured to be similar to 106 mM(-1) s(-1). After incubation with hMSCs for 12 h at a concentration of 100 mu g Fe/mL, cellular uptake of Fe3O4@SiO2 is 20-100 pg Fe/cell, which are located predominantly in the cytoplasm of cells. This level of uptake exhibits no significant influence on hMSCs' viability and differentiation. In vitro imaging of Fe3O4@SiO2-labeled hMSCs evenly distributed in agarose gel yields single cell sensitivity at 11.7 T. In vivo imaging of Fe3O4@SiO2-labeled hMSCs injected into the left brain hemisphere of nude mice yields imaging sensitivity of similar to 130 hMSCs. (C) 2013 Elsevier Ltd. All rights reserved.
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