Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 89, Issue 4, Pages 377-379Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/S1389-1723(00)88962-8
Keywords
protease; immobilization yield; polyethylene glycol; glutaraldehyde
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The effects of peptides and polyethylene glycol (PEG) on the immobilization of protease using glutaraldehyde-activated Lewatit R258-K resin were investigated. When soy peptide was added at 0.1 g/l, the activity recovery and effectiveness factor increased by 20% and 38%, respectively. When PEG4000 (0.5 g/l) was used as a protecting agent, the activity recovery and effectiveness factor were improved by 35% and 60%, respectively, leading to an increased specific activity of the immobilized enzyme. The addition of PEG4000 is suggested to improve the cost-effectiveness for the production of immobilized enzymes.
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