Journal
BIOMATERIALS
Volume 34, Issue 25, Pages 6037-6045Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.04.049
Keywords
Skeletal muscle; Stem cell; Hydrogel; bFGF
Funding
- Pioneer Research Center Program through the National Research Foundation of Korea
- Ministry of Science, ICT & Future Planning [2012-0001052]
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Skeletal muscle regeneration after sport injury is inconsistent, and complete healing without fibrosis is very important. In this study, we determined whether the combination therapy using human adipose-derived stem cells (h-ADSCs) and basic fibroblast growth factor (bFGF) incorporated into hydrogel could enhance muscle regeneration in a muscle laceration animal model. The h-ADSCs and/or bFGF hydrogels were applied to the lacerated gastrocnemius muscle. Fast twitch muscle contraction improved significantly and fibrosis decreased significantly in combined h-ADSC and bFGF-hydrogel group compared to other experimental groups. Skeletal muscle differentiation of h-ADSCs was determined by immunohistochemistry (PKH-26/MyHC co-staining) and Western blot. Our data suggested that combination therapy of h-ADSCs and bFGF hydrogel resulted in functional recovery, revascularization and reinnervation with minimal fibrosis in lacerated muscle. A combination of h-ADSCs and bFGF hydrogel can be used as a promising therapy for skeletal muscle regeneration. (C) 2013 Elsevier Ltd. All rights reserved.
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