Journal
BIOMATERIALS
Volume 33, Issue 4, Pages 999-1006Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.10.036
Keywords
Micropatterning; Antibacterial; Calcium phosphate; Bone tissue engineering; Osteoblast
Funding
- National Science Foundation [DMR 1005902]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1005902] Funding Source: National Science Foundation
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We report the use of a microfluidic 3D bone tissue model, as a high-throughput means of evaluating the efficacy of biomaterials aimed at accelerating orthopaedic implant-related wound-healing while preventing bacterial infection. As an example of such biomaterials, inkjet-printed micropatterns were prepared to contain antibiotic and biphasic calcium phosphate (BCP) nanoparticles dispersed in a poly(D,L-lactic-co-glycolic) acid matrix. The micropatterns were integrated with a microfluidic device consisting of eight culture chambers. The micropatterns immediately and completely killed Staphylococcus epidermidis upon inoculation, and enhanced the calcified extracellular matrix production of osteoblasts. Without antibiotic elution, bacteria rapidly proliferated to result in an acidic microenvironment which was detrimental to osteoblasts. These results were used to demonstrate the tissue model's potential in: (i) significantly reducing the number of biomaterial samples and culture experiments required to assess in vitro efficacy for wound-healing and infection prevention and (ii) in situ monitoring of dynamic interactions of biomaterials with bacteria as wells as with tissue cells simultaneously. (C) 2011 Elsevier Ltd. All rights reserved.
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