Journal
BIOMATERIALS
Volume 33, Issue 33, Pages 8221-8227Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.08.035
Keywords
2-Methacryloyloxyethyl phosphorylcholine polymer; Redox polymer hydrogel; Cell encapsulation; Electron-generating bacteria; Microbial-based fuel cell
Funding
- Japan Society for the Promotion of Science (JSPS) [22650107]
- Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
- Grants-in-Aid for Scientific Research [11J09692, 22650107] Funding Source: KAKEN
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Development of living cell-based devices holds great promise in many biomedical and industrial applications. To increase our understanding of the process, we investigated the biological and electrochemical properties of a redox phospholipid polymer hydrogel containing an electron-generating bacteria (Shewanella oneidensis MR-1). A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-vinylferrocene) (PMBVF), was our choice for incorporation into a hydrogel matrix that promotes encapsulation of bacteria and acts as an electron transfer mediator. This hydrogel formed spontaneously and encapsulated Shewanella in three-dimensional structures. Visual analysis showed that the encapsulated Shewanella maintained viability and metabolic activity even after long-term storage. Cyclic voltammetry measurement indicated that the PMBVF/poly(vinyl alcohol) (PMBVF/PVA) hydrogel had stable and high electron transfer efficiency. Amperometric measurement showed that the hydrogel could maintain the electron transfer efficiency even when Shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provides a mild environment for long-term bacterial survival but also maintains electron transfer efficiency from the bacteria to the electrode. We conclude that hydrogel/bacteria hybrid biomaterials, such as PMBVF/PVA/Shewanella, may find application in the fabrication of living cell-based devices. (C) 2012 Elsevier Ltd. All rights reserved.
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