Journal
BIOMATERIALS
Volume 33, Issue 31, Pages 7828-7833Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.07.026
Keywords
Dual labeling; Influenza virus; Quantum dot; Single-particle imaging; Bionanotechnology
Funding
- National Basic Research Program of China (973 Program) [2011CB933600, 2006CB933100]
- Science Fund for Creative Research Groups of NSFC [20921062]
- National Natural Science Foundation of China [21005056]
- 3551 Talent Program of the Administrative Committee of East Lake Hi-Tech Development Zone
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Many viruses invade host cells by entering the cells and releasing their genome for replication, which are remarkable incidents for viral infection. Therefore, the viral internal and external components should be simultaneously labeled and dynamically tracked at single-virus level for further understanding viral infection mechanisms. However, most of the previously reported methods have very low labeling efficiency and require considerable time and effort, which is laborious and inconvenient for researchers. In this work, we report a general strategy to high-efficiently label viral envelope and genome for single virus imaging with quantum dots (QDs) and Syto 82, respectively. It was found that nearly all viral envelopes could be labeled with QDs with superior stability, which makes it possible to realize global and long-term tracking of single virus in individual cells. Effectively labeling their genome with Syto 82, about 90% of QDs-labeled viruses could be used to monitor the viral genome signal, which may provide valuable information for deeply studying viral genome transport. This is very important and meaningful to investigate the viral infection mechanism. Our labeling strategy has advantage in commonality, convenience and efficiency, which is expected to be widely used in biological research. (C) 2012 Elsevier Ltd. All rights reserved.
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