Journal
JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION
Volume 19, Issue 2, Pages 100S-110SPublisher
AMER COLL NUTRITION
DOI: 10.1080/07315724.2000.10718071
Keywords
chemistry; analytical methods; bioavailability; content in milk; fermented milk; cheese and whey; effects of processing and storage; folate binding proteins
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In recent years, folates have come into focus due to their protective role against child birth defects, for example. neural tube defects. In addition, folates may have a protective role to play against coronary heart disease and certain forms of cancer. During the last few years most countries have established increased recommended intakes of folates, for example, between 300-400 mu g per day for adults. This review of folates in milk and dairy products compares some recent data based on high pressure liquid chromatography (HPLC) analyses and radioprotein-binding assays, with previous data based on microbiological assays. All three methods show similar ranges for folates in cow's milk 5-10 mu g per 100g, the variation being due to seasonal variations. Data on folates in fermented milk (buttermilk and yogurt) are also similar for these methods. Different starter cultures, however, might explain some of the variations in folate content and folate forms, Most cheese varieties contain between 10 mu g and 40 mu g folate per kg, with slightly higher values for whey cheese. Ripened soft cheeses may contain up to 100 mu g folate per 100g. Most previous and recent studies using HPLC indicate that 5-methyl-tetrahydrofolate (5-methyl-THF) is the major folate form in milk, but more studies are needed concerning folate forms in other, especially fermented dairy products. Relatively new data on actual concentrations in different dairy products show folate-binding proteins (FBP) to occur in unprocessed mill;, bur also in pasteurised milli spray-dried skim milk powder and whey. In contrast, UHT milk, fermented milk and most cheeses only contain low levels or trace amounts.
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