Journal
BIOMATERIALS
Volume 33, Issue 13, Pages 3515-3528Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.01.040
Keywords
Ti6Al4V alloy; BMP2; Layer-by-layer assembly technique; Extracellular microenvironment; Mesenchymal stem cells; Osteogenesis
Funding
- China Ministry of Science and Technology [2009CB930000]
- Natural Science Foundation of China [11032012, 51173216]
- Fundamental Research Funds for the Central Universities [CDJXS10232211]
- Fok Ying Tung Education Foundation [121035]
- Natural Science Foundation of Chongqing Municipal Government (CSTC) [2011jjjq10004, 2010AB5116, JJA10056, 2008AB5129]
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To mimic the extracellular microenvironment of bone, a bioactive multilayered structure of gelatin/chitosan pair, containing bone morphogenetic protein 2(BMP2) and fibronectin (FN), was constructed onto Ti6Al4V surface via a layer-by-layer assembly technique. The successful fabrication of multilayered structure was confirmed by contact angle measurement, field emission scanning electron microscopy (FE-SEM) and confocal laser scanning microscopy (CLSM), respectively. Bioactive BMP2 released in a sustained manner along with the degradation of multilayered structure. MSCs grown onto the multilayer coated TC4 substrates displayed significantly higher (p < 0.01 or p < 0.05) production levels of alkaline phosphatase (ALP), mineralization and genes expressions of runt related transcription factor 2 (Runx2), osterix, osteocalcin (OC), osteopontin (OPN). ALP and collagen type 1(Coll) compared to the controls after culture for 7 days and 21 days, respectively. More importantly, MicroCT analysis and histological observations demonstrated that the multilayer coated Ti6Al4V implants in vivo promoted the bone density and new bone formation around them after implantation for 4 weeks and 12 weeks, respectively. The results indicated that Ti6Al4V coated with biofunctional multilayers was beneficial for osteogenesis and integration of implant/bone. The study therefore presents an alternative to fabricate bio-functionalized Ti6Al4V-based implants for potential application in orthopedics field. (C) 2012 Elsevier Ltd. All rights reserved.
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