Journal
BIOMATERIALS
Volume 33, Issue 8, Pages 2439-2448Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.11.075
Keywords
Dextran; MRI; Progenitor cell; Transplantation
Funding
- Ministry of Health, Labour, and Welfare of Japan [18A-2]
- Japan Association for the Advancement of Medical Equipment
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A water-soluble magnetic resonance imaging (MRI) contrast agent, Dextran mono-N-succinimidyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate-gadolinium(3+) (Dex-DOTA-Gd3+), was shown to enable monitoring of the anatomical migration and the survival period of transplanted stem cells for up to 1 month. Gadolinium molecules in the cells were rapidly eliminated from the site and excreted upon cell death. Endothelial progenitor cells (EPCs) transplanted into the inguinal femoral muscle of rats migrated distally through the knee in rats after hindlimb ischemia but did not migrate in non-ischemic rats. Interestingly, the survival period of transplanted EPCs was notably prolonged in the ischemic limb, indicating that EPCs are required by the ischemic tissues and that the fate of transplanted EPCs was affected by the disease. Compared to the commonly used particle type of MRI contrast agents, the system described in this study is expected to be invaluable to help clarifying the process of stem cell transplantation therapy. (C) 2011 Elsevier Ltd. All rights reserved.
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