Journal
BIOMATERIALS
Volume 33, Issue 27, Pages 6430-6437Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.05.056
Keywords
Biosensor; microRNA-1; Molecular beacon; Reporter gene; Myogenic differentiation; Molecular imaging
Funding
- Nuclear Research & Development Program of National Research Foundation of Korea (NRF)
- Ministry of Education, Science & Technology (MEST) [2010-0018684]
- Bio & Medical Technology Development Program of the National Research Foundation (NRF)
- Korean government (MEST) [2012-0006097]
- National Research Foundation of Korea [2010-0018684] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Dual optical microRNA (miRNA) imaging systems, bioluminescent reporter gene (a signal-off mechanism)or fluorescent molecular beacon (MB) (a signal-on mechanism)-based miRNA imaging system, have individually allowed us to sense miRNA biogenesis in a noninvasive and iterative manner. Both of these imaging systems have shortcomings with respect to image quality. Therefore, we designed a dual optical imaging systems by simultaneous imaging of a miRNA-1 reporter gene (CMV/Gluc/3xPT_miR-1) and miRNA-1 MB in a single cell to overcome these limitations and used it to visualize miRNA-1, a highly expressed miRNA in cardiac and skeletal muscle. During myogenic differentiation of C2C12 cells, the bioluminescence intensity from CMV/Gluc/3xPT_miR-1 revealed a miRNA-1-dependent gradual decrease and the fluorescence intensity from miR-1 MB demonstrated a miRNA-1-dependent gradual increase both in vitro and in vivo. The dual miRNA-1 imaging systems, which provides the complementary imaging information about miRNA biogenesis, could be useful to sense miRNA expression during various biologic processes. (c) 2012 Elsevier Ltd. All rights reserved.
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