Lactoferrin-conjugated superparamagnetic iron oxide nanoparticles as a specific MRI contrast agent for detection of brain glioma in vivo

A specific contrast agent for magnetic resonance imaging (MRI) is crucial to brain tumor patients for the surgical operation or the postoperative radiology This study explored lactoferrin-conjugated superparamagnetic iron oxide nanoparticles (Lf-SPIONs) as an MRI contrast agent for the detection of brain gliomas in vivo The hydrodynamic diameter of about 75 nm saturation magnetization of 51 emu/g Fe and T-2 relaxivity of 75 6 mM(-1)S(-1) of the Lf-SPIONs suggested its applicability for MRI Using a rat model of C6 glioma Lf-SPIONs provided a better picture or more sensitivity to depict brain glioma on MR images than that of SPIONs Significantly enhanced T-2-weighted images of brain glioma were documented in vivo with Lf-SPIONs until 48 h after injection Moreover Lf-SPIONs were clearly observed around vascular region of the tumor slices after 48 h High level expression of Lf receptors was confirmed in brain tumor tissues by RT-PCR and Western Blot compared to normal brain tissues These findings suggested that Lf-SPIONs could be potentially employed as a sensitive and specific MRI contrast agent in the diagnosis of brain glioma (C) 2010 Elsevier Ltd All rights reserved