Journal
BIOMATERIALS
Volume 32, Issue 3, Pages 787-796Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.09.046
Keywords
Peripheral nerve injury; Nerve tissue engineering; Nerve regeneration; Bone marrow stromal cell; Chitosan; Biomaterials
Funding
- University of Hong Kong
- Dr Cheng Yu-Tung Fellowship
- LKS Faculty of Medicine The University of Hong Kong
- Hong Kong Research Grants Council [GRF 777810]
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Autologous nerve grafts have been the gold standard for treatment of peripheral nerve defects that exceed the critical gap length To address issues of limited availability of donor nerves and donor site morbidity we have fabricated chitosan conduits and seeded them with bone marrow stromal cell (BMSC)-derived Schwann cells as an alternative The derived Schwann cells used were checked for fate commitment The conduits were tested for efficacy in bridging the critical gap length of 12 mm in sciatic nerves of adult rats By three months post-operation mid-shank circumference nerve conduction velocity average regenerated myelin area and myelinated axon count in nerves bridged with BMSC-derived Schwann cells were similar to those treated with sciatic nerve-derived Schwann cells (p > 0 05) but significantly higher than those bridged with PBS-filled conduits (p < 0 05) Evidence is thus provided in support of the use of chitosan conduits seeded with BMSC-derived Schwann cells to treat critical defects in peripheral nerves This provides the basis to pursue BMSC as an autologous source of Schwann cells for transplantation therapy in larger animal species (C) 2010 Elsevier Ltd All rights reserved
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