Journal
BIOMATERIALS
Volume 32, Issue 9, Pages 2334-2341Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.11.059
Keywords
Liposome; Diagnostics; Positron emission tomography (PET); Tumor accumulation; Remote loading
Funding
- Danish Strategic Research Council (NABIIT) [2106-07-0033]
- Technical University of Denmark (DTU)
- AP Moller Foundation
- Danish National Advanced Technology Foundation
- Novo Nordisk Foundation
- Lundbeck Foundation
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We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide (Cu-64) using a new ionophore, 2-hydroxyquinoline, to carry Cu-64(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 +/- 1.6%) and retention stability (>99%), which makes the Cu-64-liposomes highly applicable as PET imaging agents. We show the utility of the Cu-64-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications. (C) 2010 Elsevier Ltd. All rights reserved.
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