Journal
BIOMATERIALS
Volume 32, Issue 33, Pages 8584-8592Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.07.091
Keywords
Graphene; Gold nanoparticle; Layer-by-layer assembly; DNA sensor; Lab-on-chip
Funding
- National Natural Science Foundation of China [21075116, 20935003, 20820103037]
- 973 Project [2009CB930100, 2011CB911002, 2010CB933600]
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In this article, we demonstrate the use of graphene-mesoporous silica-gold NP hybrids (GSGHs) as an enhanced element of the integrated sensing platform for the ultra-sensitive and selective detection of DNA by using strand-displacement DNA polymerization and parallel-motif DNA triplex system as dual amplifications. We find that the present new sensing strategy based on GSGHs is able to detect target DNA with a fairly high detection sensitivity of 10 fM through the hybridization of duplex DNA to the acceptor DNA for the formation of parallel-motif DNA triplex on the multilayer film (containing GSGHs and redox probe) modified functional interface, and even has a good capability to investigate the single nucleotide polymorphisms (SNPs). The detection limit for target DNA is about two orders of magnitude lower than that of graphene-based DNA electrochemical impedance spectroscopy (EIS) sensor (6.6 pM), four orders of magnitude lower than those of graphene-based DNA sensors coupled with fluorescent assay (100 pM and 1 nM) and five orders of magnitude lower than those of field effect transistor (FET)-based assays (1 nM and 2 nM). Most importantly, our present sensing system can also be facilely achieved in the ITO electrode array, which is of paramount importance for possible multiplex analysis in lab-on-chip. (C) 2011 Elsevier Ltd. All rights reserved.
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