Journal
BIOMATERIALS
Volume 32, Issue 3, Pages 879-889Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.09.052
Keywords
Poly(epsilon caprolactone); Gene therapy; Degradable polymers; Poly(2 (dimethylamino ethyl)methacrylate); Amphiphilic copolymers
Funding
- National Key Basic Research Program of China [2009CB930200]
- National Grand Program on Key Infectious Disease Control [2008ZX10001-015-10]
- National Natural Science Foundation of China [30970784, 30772007]
- Tianjin Natural Science Foundation [09JCYBJC13800]
- NIH/NCRR/RCMI [2 G12 RR003048]
- Specialized Research Fund for the Doctoral Program of Higher Education [20090032120013]
- NATIONAL CENTER FOR RESEARCH RESOURCES [G12RR003048] Funding Source: NIH RePORTER
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A group of amphiphilic cationic polymers methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD) were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs) The structures of these amphiphilic cationic polymers were characterized by H-1 NMR measurement The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5) The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection (C) 2010 Elsevier Ltd All rights reserved
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