Journal
BIOMATERIALS
Volume 32, Issue 3, Pages 819-831Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.09.057
Keywords
Inverted colloidal crystal; Laminin derived peptide; Bone marrow stromal cell; Neuron; Differentiation
Funding
- National Science Council of the Republic of China
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This study presents the effect of pore regularity on the preservation and differentiation of bone marrow stromal cells (BMSCs) Scaffolds with interconnected pores of Inverted colloidal crystal (ICC) geometry were prepared by infiltrating chitosan gelatin gels into the interstices of self-assembled microspheres which were later dissolved with a solvent In addition the pore surfaces were grafted with two laminin-derived peptides (LDP) The experimental results revealed that the number of BMSCs in ICC scaffolds could increase 2 7-fold after cultivation over 7 days Moreover the distribution of cultured BMSCs in ICC scaffolds was quite uniform as compared with freeform scaffolds ICC scaffolds could preserve 63% phenotypic BMSCs in average and freeform scaffolds 56% The grafted LDP enhanced the adhesion efficiency of BMSCs in ICC scaffolds (about 70-75%) and produced NeuN-positive cells A further induction with neuron growth factor could guide the differentiation of BMSCs toward mature neurons in LDP-grafted ICC scaffolds The controlled topography of ICC structure and surface LDP can be promising in the cultivation of BMSCs and neural regeneration (C) 2010 Elsevier Ltd All rights reserved
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