Ultrasensitive electrochemical immunoassay for BRCA1 using BMIM•BF4-coated SBA-15 as labels and functionalized graphene as enhancer

BRCA1 is an anti-oncogene in women, who are genetically predisposed to breast and ovary cancer. The detection of BRCA1 can offer an opportunity to characterize the function of genetic features in breast and ovarian cancer and to screen breast or ovarian cancer patients. In this study, we designed a new label and fabricated a novel sandwich-type electrochemical immunoassay for the ultrasensitive detection of BRCA1 Horseradish peroxidase (HRP) was entrapped in the pores of amino-group functionalized SBA-15 and the secondary antibody (Ab(2)) combined with SBA-15 by covalent bond. Ionic liquid (IL) was added into the mixed solution of SBA-15/HRP/Ab(2) and application of IL increased the electrochemical activity of HRP and promoted electron transport. The synergistic effect between IL SBA-15, Ab(2) and HRP could retain the bioactivity of HRP and Ab(2). The sensitivity of the sandwich-type immunosensor using SBA-15/HRP/Ab(2)/BMIM. BF4 as labels for BRCA1 detection was much higher than that using either SBA-15/HRP/Ab(2) or SBA-15/Ab(2) as labels. Under optimal conditions, the electrochemical immunoassay exhibited a wide working range from 0.01 to 15 ng/mL with a detection limit of 4.86 pg/mL BRCA1. The precision, reproducibility, and stability of the immunoassay were acceptable. (C) 2010 Elsevier Ltd. All rights reserved.