Journal
BIOMATERIALS
Volume 32, Issue 6, Pages 1455-1464Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.10.057
Keywords
Biosensor; Gold; Nanoparticle; Scaffold; Self assembly; Surface modification
Funding
- Bio-oriented Technology Research Advancement Institution (BRAIN) of Japan
- Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)
- Japan Science and Technology Agency (JST)
- Ministry of Health & Welfare, Republic of Korea [A062254]
- Korea Science and Engineering Foundation [2008-03876]
- National Research Foundation of Korea [2008-03876] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Grants-in-Aid for Scientific Research [23710143, 22650111] Funding Source: KAKEN
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To enhance the sensitivities and antigen-binding capacities of immunosensors, oriented immobilization of antibodies on the surface of the sensor chip is critical, but to date, this has not been adequately achieved. We describe a way of adsorbing immunoglobulin (Ig) proteins onto 32-nm bio-nanocapsules (BNCs) through IgG Fc-binding domains derived from Staphylococcus aureus protein A (ZZ-BNC). This arrangement permits similar to 60 molecules of mouse total IgG bind to ZZ-BNC and all the IgG Fv regions to be displayed outwardly for the effective binding of antigens. ZZ-BNCs adsorbed onto the gold surface of the sensor chip of the quartz crystal microbalance (QCM) could markedly enhance the sensitivity and antigen-binding capacity of the chip. On the sensor chip of surface plasmon resonance (SPR), antibodies on the ZZ-BNCs showed higher affinities to each antigen than those on protein A. The BNC-coated sensor chip is very stable, and should prove useful for various immunosensor applications due to oriented immobilization of antibodies. (C) 2010 Elsevier Ltd. All rights reserved.
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