Journal
BIOMATERIALS
Volume 32, Issue 8, Pages 2133-2140Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.11.034
Keywords
Quantum nanospheres; Biocompatibility; Cell imaging; Biological probes
Funding
- 863 project [2006AA03Z302]
- National Basic Research Program of China [2007CB936000]
- National Natural Science Foundation of China [30900338, 20725516, 30425020, 90813010, 20873175, 20805055, 90913014, 51072126]
- Ministry of Health [2009ZX10004-301]
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A new class of fluorescent quantum nanospheres (QNs) is directly achieved in aqueous phase through a facile one-pot microwave irradiation (MWI) strategy. Multi-color QNs with maximum emission wavelengths ranging from 525 to 610 nm and PLQY of 30-60% are facilely prepared through this new MWI strategy. In addition to strong fluorescence, these QNs possess excellent photostability, preserving similar to 90% of the original intensity after 70 min high-power UV irradiation (100 W Xeon lamp). In sharp contrast, the fluorescence of CdTe/CdS/ZnS core-shell-shell quantum dots (QDs), recognized as established fluorescent probes with robust photostability, decrease to similar to 50% under the same conditions. Besides, cytotoxicity assessment demonstrates that the prepared QNs exhibit favorable cytocompatibility to K562 cells with high concentration (3 mu mol) and long-time incubation (24 h). Furthermore, cellular imaging results demonstrate that the as-prepared QNs are remarkably efficacious for long-term and high-specificity immunofluorescent cellular labeling, and multi-color cell imaging. Our systematical investigation clearly shows that these high-performance QNs may serve as practical and powerful tools for various biological researches, such as in vivo and in vitro imaging. (C) 2010 Elsevier Ltd. All rights reserved.
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