Journal
BIOMATERIALS
Volume 31, Issue 25, Pages 6425-6435Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.04.064
Keywords
Extracellular matrix; Collagen translocation; Cell migration; Cell spreading; Mechanoregulation
Funding
- National Institutes of Health [GM31321]
- UT Southwestern
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM031321, R37GM031321, R01GM096070] Funding Source: NIH RePORTER
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In three dimensional collagen matrices, cell motile activity results in collagen translocation, cell spreading and cell migration. Cells can penetrate into the matrix as well as spread and migrate along its surface. In the current studies, we quantitatively characterize collagen translocation, cell spreading and cell migration in relationship to collagen matrix stiffness and porosity. Collagen matrices prepared with 1-4 mg/ml collagen exhibited matrix stiffness (storage modulus measured by oscillating rheometry) increasing from 4 to 60 Pa and matrix porosity (measured by scanning electron microscopy) decreasing from 4 to 1 mu m(2). Over this collagen concentration range, the consequences of cell motile activity changed markedly. As collagen concentration increased, cells no longer were able to cause translocation of collagen fibrils. Cell migration increased and cell spreading changed from dendritic to more flattened and polarized morphology depending on location of cells within or on the surface of the matrix. Collagen translocation appeared to depend primarily on matrix stiffness, whereas cell spreading and migration were less dependent on matrix stiffness and more dependent on collagen matrix porosity. (C) 2010 Elsevier Ltd. All rights reserved.
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