Journal
BIOMATERIALS
Volume 31, Issue 6, Pages 1080-1084Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.10.041
Keywords
Complex coacervate; Hyaluronic acid; Mussel adhesive protein; fp-151-RGD; MC-3T3; Biocoating
Funding
- National Science Foundation [DMR05-20415]
- National Institutes of Health [R01-DE018468]
- Otis-Williams Postdoctoral Fellowship in Bioengineering, UCSB (via Santa Barbara Foundation)
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Many biological polyelectrolytes are capable of undergoing a fluid-fluid phase separation known as complex coacervation. Coacervates were prepared using hyaluronic acid (HA) and a recombinant fusion protein consisting of mussel adhesive motifs and the RGD peptide (fp-151-RGD). The low interfacial energy of the coacervate was exploited to coat titanium (Ti), a metal widely used in implant materials. The coacervate effectively distributed both HA and fp-151-RGD over the Ti surfaces and enhanced osteoblast proliferation. Approximately half of total fp-151-RGD and HA in the solution transferred to the titanium surface within 2 h. Titanium coated with coacervates having high residual negative surface charge showed the highest cell proliferation of preosteoblast cells (MC-3T3) compared to the treatments tested. Indeed, MC-3T3 cells on complex coacervate coated titanium foils exhibited over 5 times greater cell proliferation than bare, HA coated or fp-151-RGD coated titanium. (C) 2009 Elsevier Ltd. All rights reserved.
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