Journal
BIOMATERIALS
Volume 31, Issue 28, Pages 7239-7249Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.05.040
Keywords
Tendon; Tissue engineering; Knitted silk-collagen sponge scaffold; rhSDF-1 alpha; Regeneration
Funding
- International S&T Cooperation Program of China (ISTCP) [2008DFB30090]
- Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents
- Foundation of Zhejiang Provincial Key Medical Discipline (Medical Tissue Engineering), Zhejiang Province [Y2080141]
- Program for New Century Excellent Talents in University [NCET-08-0487]
Ask authors/readers for more resources
This study developed a bioactive knitted silk-collagen sponge scaffold by incorporation of exogenous SDF-1 alpha, to enable selective migration and homing of cells for in situ tendon regeneration. With in vitro studies, it was observed that CXCR4 gene expression and migration of bone mesenchymal stromal cells and hypo-dermal fibroblasts were more sensitive to exogenous SDF-1 alpha, while expression of tendon repair gene markers by hypo-dermal fibroblasts and Achilles tendon fibroblasts were more sensitive to exogenous SDF-1 alpha. With a rat Achilles tendon injury model, exogenous SDF-1 alpha was shown to reduce infiltration of inflammatory cells and enhance migration of fibroblast-like cells into the scaffold at 4 days and 1 week post-surgery. After 4 weeks, SDF-1 alpha treated tendon had increased expression of tendon repair gene markers and endogenous SDF-1 alpha, exhibited more physiological microstructures with larger diameter collagen fibrils, and had better biomechanical properties than the control group. Hence, our bioactive scaffold improved efficacy of tendon regeneration by increasing the recruitment of fibroblast-like cells, enhancing local endogenous SDF-1 alpha and tendon extracellular matrix production, and decreasing accumulation of inflammatory cells. Incorporation of SDF-1 alpha within a knitted silk-collagen sponge scaffold can therefore be a practical application for tendon tissue engineering. (C) 2010 Published by Elsevier Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available