Journal
BIOMATERIALS
Volume 31, Issue 7, Pages 1798-1804Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.10.034
Keywords
Bioreducible polymer; Gene transfer; Guanidinylation; Cytotoxicity; Nuclear localization ability
Funding
- NIH [DK 077703]
- Ministry of Education, Science and Technology, Korea [WCU 200900000000024]
- National Research Foundation of Korea [R33-2008-000-10036-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Guanidinylated bioreducible polymer (GBP) was developed for gene delivery systems utilizing cellular penetrating ability of guanidine groups. GBP could retard pDNA from a weight ratio of 5 completely in agarose gel electrophoresis but pDNA was released from GBP polyplexes even at a weight ratio of 20 in reducing condition (2.5 mm DTT) due to their biodegradation. GBP also could construct 200 nm-sized and positively charged (similar to 30 mV) polyplex nanoparticles with pDNA. The cytotoxicity of GBP was found to be minimal and GBP showed about 8 folds improved transfection efficiency than a scaffold polymer, poly(cystaminebisacrylamide-diaminohexane) (poly(CBA-DAH)) and even higher transfection efficiency than PEI25k in mammalian cell lines. Its high cellular uptake efficiency (96.1%) and strong nuclear localization ability for pDNA delivery due to the structural advantage of bioreducible polymer and guanidine groups were also identified, suggesting GBP is a promising candidate for efficient gene delivery systems. (C) 2009 Elsevier Ltd. All rights reserved
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