Journal
BIOMATERIALS
Volume 31, Issue 36, Pages 9431-9437Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.08.071
Keywords
Endothelial cells; Nanofibers; Electrospinning; Mussel adhesives; Poly(dopamine); Vascular tissue scaffold
Funding
- National Research Foundation (NRF) via National Research Laboratory (NRL) [ROA-2008-000-20041-0]
- Converging Research Center [2009-0082276]
- Bio-Green 21 Program [20070301034038]
- KAIST Institute for the NanoCentury (KINC) Republic of Korea
- National Research Foundation of Korea [2010-50207] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [20070301034038] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The endothelialization of prosthetic scaffolds is considered to be an effective strategy to improve the effectiveness of small-diameter vascular grafts We report the development of a nanofibrous scaffold that has a polymeric core and a shell mimicking mussel adhesive for enhanced attachment proliferation and phenotypic maintenance of human endothelial cells Polycaprolactone (PCL) was chosen as a core material because of its good biodegradability and mechanical properties suitable for tissue engineering PCL was electrospun into nanofibers with a diameter of approximately 700 nm and then coated with poly (dopamine) (PDA) to functionalize the surface of PCL nanofibers with numerous catechol moieties similar to mussel adhesives in nature The formation of a PDA ad-layer was analyzed using multiple techniques including scanning electron microscopy Raman spectroscopy and water contact angle measurements When PDA-coated PCL nanofibers were compared to unmodified and gelatin-coated nanofibers human umbilical vein endothelial cells (HUVECs) exhibited highly enhanced adhesion and viability increased stress fiber formation and positive expression of endothelial cell markers (e g PECAM-1 and vWF) (C) 2010 Elsevier Ltd All rights reserved
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