Journal
BIOMATERIALS
Volume 31, Issue 31, Pages 8097-8105Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.07.015
Keywords
siRNA; Graft copolymer; Polyion complex; Poly(aspartate); Endosome escape
Funding
- Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [21659299] Funding Source: KAKEN
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An siRNA-grafted polymer through disulfide linkage was prepared to improve the physicochemical properties and transfection efficacies of the polyion complexes (PICs) as a nanocarrier of siRNA. The siRNA-grafted polymer formed stable PICs due to its larger numbers and higher density of anionic charges compared with monomeric siRNA, leading to effective internalization by cultured cells. Following the endosomal escape of the PIC, the disulfide linkage of the siRNA-grafted polymer allowed efficient siRNA release from the PIC under intracellular reductive conditions. Consequently, the PIC from the siRNA-grafted polymer showed a potent gene silencing effect without cytotoxicity or immunogenicity, demonstrating a promising feature of the siRNA-grafted polymer to construct the PIC-based nanocarrier for in vivo siRNA delivery. (c) 2010 Elsevier Ltd. All rights reserved.
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