Journal
BIOMATERIALS
Volume 31, Issue 7, Pages 1683-1690Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.11.056
Keywords
Tissue engineering; Photolithography; Curvature; Cell culture
Funding
- NIH [DP2-OD004346-01, DP2-OD004346-01S1]
- National Science Foundation IGERT [DGE-0549350]
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We describe the use of conventional photolithography to construct three dimensional (3D) thin film scaffolds and direct the growth of fibroblasts into three distinct and anatomically relevant geometries: cylinders, spirals and bi-directionally folded sheets. The scaffolds were micropatterned as two dimensional sheets which then spontaneously assembled into specific geometries upon release from the underlying substrate. The viability of fibroblasts cultured on these self-assembling scaffolds was verified using fluorescence microscopy; cell morphology and spreading were studied using scanning electron microscopy. We demonstrate control over scaffold size, radius of curvature and folding pitch, thereby enabling an attractive approach for investigating the effects of these 3D geometric factors on cell behaviour. (C) 2009 Elsevier Ltd. All rights reserved.
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