Journal
BIOMATERIALS
Volume 31, Issue 32, Pages 8313-8322Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.07.066
Keywords
Alignment; Collagen; Cornea; Scaffold
Funding
- Hospices Civils de Lyon
- CNRS
- Universite Claude Bernard Lyon I
- European Union [NMP2-CT-2003-50417]
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We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemicorneas (stroma + epithelium), using primary human keratocytes and limbal stem cell derived human keratinocytes. On the surface of these hemi-corneas, a well-differentiated epithelium was formed, as determined both histologically and ultrastructurally and by the expression of characteristic markers. Within the stroma, the keratocytes aligned with the directions of the fibrils in the scaffold and synthesized a new extracellular matrix with typical collagen markers and small, uniform diameter fibrils. Finally, in vivo experiments using a rabbit model showed that these orthogonally oriented multi-layer scaffolds could be used to repair the anterior region of the stroma, leading to re-epithelialization and recovery of both transparency and ultrastructural organization. (C) 2010 Elsevier Ltd. All rights reserved.
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