Journal
BIOMATERIALS
Volume 31, Issue 19, Pages 5091-5099Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.02.076
Keywords
Polyethylene terephthalate; Contact guidance; Directional angiogenesis; Microvessel formation; Multi-cellular lumen
Funding
- Canadian Foundation for Innovation/Ministere de l'Education du Quebec [7918]
- National Sciences and Engineering Research Council of Canada (NSERC)
- Universite de Sherbrooke
- IDB
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In the present study, polymer monofilaments were embedded in fibrin seeded with human umbilical vein endothelial cells (HUVEC) to guide HUVEC attachment and migration in order to form oriented vessel-like structures between adjacent monofilaments. Histology and fluorescent fibrin experiments confirmed that microvessel-like structures, which were developing between polymer monofilaments embedded in fibrin, contained a lumen. The effect of human fibroblasts and growth factors (VEGF and bFGF) over the microvessel formation process was tested. The effects of VEGF and bFGF were dose-dependent. The effect of VEGF was optimum at the lower concentration tested (2 ng/mL), while that of bFGF was optimum at the higher tested concentration (20 ng/mL). Furthermore, the use of fibroblasts significantly improved the maturation of the microvessels compared to control and to samples cultured with VEGF and bFGF. (C) 2010 Elsevier Ltd. All rights reserved.
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