Journal
BIOMATERIALS
Volume 31, Issue 35, Pages 9266-9272Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.08.067
Keywords
Secondary structure; Peptide; Thermally responsive material; Hydrogel; Chondrocyte; Cell culture
Funding
- MEST of Korea [2010-0000832, 313-2008-2-C00590, 2010-0001487, 2010-0002170]
- Ministry of Knowledge Economy, Republic of Korea [K00060-282]
- Korea Evaluation Institute of Industrial Technology (KEIT) [K0006028] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [313-2008-2-C00590, 2008-2000033, 2008-0057903, 2009-0080447] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In the search for a cell-instructive or cell-interactive artificial extracellular matrix, synthetic hydrogels have been extensively investigated to apply three-dimensional (3D) cell culture and tissue engineering. Here, we are reporting a reverse thermal gelling L/DL-polyalanine block copolymer aqueous solution for chondrocyte 3D culture. The polymer aqueous solution undergoes sol-to-gel transition as the temperature increases, thus forming a 3D cell encapsulating scaffold in situ at 37 degrees C. In particular, the fraction of the beta-sheet structure of the polyalanine dictated the population and thickness of fibrous nanostructure of the hydrogel, which in turn affected the proliferation and protein expression of the encapsulated chondrocytes. As an injectable tissue engineering system of chondrocytes, very promising results were confirmed for nude mice, using the current polypeptide aqueous solution. This paper not only provides important clues in designing an artificial extracellular matrix but also proves the significance of thermal gelling polypeptide as a minimally-invasive tissue engineering scaffold. (C) 2010 Elsevier Ltd. All rights reserved.
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