Journal
BIOMATERIALS
Volume 31, Issue 9, Pages 2695-2700Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.12.021
Keywords
Biomimetic material; FTIR; Honeybee; Apis mellifera; Recombinant protein; Silk
Funding
- CSIRO
- Australian Grains Research and Development Corporation
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Transgenic production of silkworm and spider silks as biomaterials has posed intrinsic problems due to the large size and repetitive nature of the silk proteins In contrast the silk of honeybees (Apis mellifera) is composed of a family of four small and non-repetitive fibrous proteins We report recombinant production and purification of the four full-length unmodified honeybee silk proteins in Escherichia colt at substantial yields of 0 2-2 5 g/L. Under the correct conditions the recombinant proteins self-assembled to reproduce the native coiled coil structure. Using a simple biomimetic spinning system we could fabricate recombinant silk fibers that replicated the tensile strength of the native material (C) 2009 Elsevier Ltd. All rights reserved.
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