Journal
METABOLIC ENGINEERING
Volume 2, Issue 2, Pages 140-148Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/mben.2000.0146
Keywords
metabolic flux analysis; energy requirement; Streptomyces coelicolor; nutrient limitation
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Funding
- Ministry of Higher Education of the Islamic Republic of Iran
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Metabolic flux analysis was applied to Streptomyces coelicolor continuous culture data obtained under nitrogen, phosphate, sulfate, and potassium limitations. The metabolic reaction network involved more than 200 reactions describing the major pathways as well as the secondary metabolism for the production of actinorhodin and excretion of certain metabolites. Linear programming was used for the optimization of specific growth rates and energy requirements. Two types of specific growth rates, stoichiometric and theoretical, were defined, maximized, and compared in order to investigate the microbial potential. Potassium limitation led to the largest and nitrogen limitation to the smallest difference between the stoichiometric and theoretical specific growth rates. Although the value of the maximum theoretical specific growth rate was close to that of the experimental specific growth rate with potassium limitation, this difference was the largest in the case of nitrogen limitation. Energy requirements during different nutrient limitations were also investigated. The model indicated that although the highest actinorhodin production rate was with nitrogen limitation, this was accompanied with the undesired excretion of certain metabolites. (C) 2000 Academic Press
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