Journal
BIOMATERIALS
Volume 30, Issue 8, Pages 1482-1491Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.11.040
Keywords
ECM (extracellular matrix); Biocompatibility; Macrophage; Monocyte; Wound healing
Funding
- NIBIB NIH HHS [F31 EB007914-01A2, F31 EB007914] Funding Source: Medline
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Recently, macrophages have been characterized as having an MI or M2 phenotype based on receptor expression, cytokine and effector molecule production, and function. The effects of macrophage phenotype upon tissue remodeling following the implantation of a biomaterial are largely unknown. The objectives of this Study were to determine the effects of a cellular component within an implanted extracellular matrix (ECM) Scaffold upon macrophage phenotype, and to determine the relationship between macrophage phenotype and tissue remodeling. Partial-thickness defects in the abdominal wall musculature of Sprague-Dawley rats were repaired with autologous body wall tissue, acellular allogeneic rat body wall ECM, xenogeneic pig urinary bladder tissue, or acellular xenogeneic pig urinary bladder ECM. At 3, 7, 14, and 28 days the host tissue response was characterized using histologic, immunohistochemical, and RT-PCR methods. The acellular test articles were shown to elicit a predominantly M2 type response and resulted in constructive remodeling, while those containing a cellular component. even an autologous cellular component, elicited a predominantly MI type response and resulted in deposition of dense connective tissue and/or scarring. We conclude that the presence of cellular material within in ECM scaffold modulates the phenotype of the macrophages participating in the Host response following implantation, and that the phenotype of the macrophages participating in the host response appears to be related to tissue remodeling Outcome. (C) 2008 Elsevier Ltd. All rights reserved.
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