Journal
BIOMATERIALS
Volume 30, Issue 21, Pages 3503-3512Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.03.025
Keywords
Self-assembled monolayers; XPS; PM-RAIRS; Bacteria; Magainin; Antibacterial surfaces
Funding
- PNIR Biofilms CNRS
- French ANR SURFANBAC [BLAN06-1_134847]
Ask authors/readers for more resources
An antibacterial peptide, Magainin I, was covalently bound to a mixed 11-mercaptoundecanoic acid (MUA) and 6-mercaptohexanol (C6OH) (ratio 1:3) Self-Assembled Monolayer (SAM) on gold surfaces. Each step of the surface functionalization was characterized by Polarization Modulation Reflection Absorption InfraRed Spectroscopy (PM-RAIRS) and X-ray Photoelectron Spectroscopy (XPS). The antibacterial activity of the anchored Magainin was tested against three Gram-positive bacteria (Listeria ivanovii, Enterococcus faecalis and Staphylococcus aureus), and the results revealed that the adsorbed Magainin I reduced by more than 50% the adhesion of bacteria at the surface, together with the killing of the bacteria that nonetheless adhered to the surface. No release of the peptide was observed upon contact with the bacterial suspension; the activity has proven to be persistent overtime, up to six months after the first use. (C) 2009 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available